Flow Cytometer

High-speed Cell Sorter

Highspeed CellsorterFast & precise cell sorting.
EQ’s high-speed cell sorter sets standards for cell sorting with functionality, speed and improved sensitivity. It allows for fast separation of cells according to their metabolic, physiological or other characteristics. It is suitable for selection and cloning of high-producing cell lines for cell line development or for isolation of patient cells for medical applications. In combination with other EQ instruments, it can be used to analyze the metabolome or proteome of defined subpopulations within a population.

TECHNICAL INFORMATION
MoFlo Astrios. Beckman Coulter. Obtained 2012.
The MoFlo Astrios is a high-speed cell sorter with improved sensitivity. Equipped with five lasers, the instrument can analyze up to 13 size and fluorescence parameters. Isolation of cells with defined properties for subsequent culture or further analysis is possible both in bulk and into microtiter plates and allows in-depth studies of cellular phenotypes. Biosafety aerosol containment enables sterile sorting of up to six subpopulations for cell line development.

 

Cell Sorter SONY SH800S

Cell sorting made simple.

It permits sorting a wide range of cell sizes for many applications using the 70-μm, 100-μm and 130-μm microfluidic sorting chips. To make sorting less subjective, more precise and easier to perform there is a novel chip based design which is fully integrated with comprehensive fluidics controls and advanced automation for set-up, acquisition, sort and anaysis.
The system software is intuitive and supports sorting into tubes and 96 – and 384 – well plates. It generates FCS 3.0 and FCS 3.1 files that can be exported to third party analysis tools.
Sony SH800S is used for non- clinical research and a class 1 laser product.

TECHNICAL INFORMATION
Sony SH800S. Obtained 2018.
This cell sorter ist flexible and can support a variety of applications for individual and core labs. Cross-contamination is often a problem, therefore e-beam sterilized consumables can be used optional to replace the sample line and sorting chip.
For the personal and product safety it provides an class A2 Level II biosafety cabinet with international standards.
The optical design offers up to four collinear excitation lasers (488 nm, 405nm, 561nm and 638nm) and six fluorescence detectors. The six free-form PMTs enable detection of fluorescence signals from any laser based on filter selection.

 

Cytoflex S
Qualitative and quantitative measurement of biological and physical properties of cells and other particles.
These properties are measured when the cells pass through one or multiple laser beams in a single-file. The CytoFLEX flow cytometer can perform up to 13 color marker analysis. Infrared laser provides untouched fluorescent channels with minimal spectral overlap into traditional channels. With the fixed integrated optics and quartz flow cell design this Flow sizer is easy to port away. Avalanche Photodiode (APD) detectors provide unmatched red sensitivity and unique compensation workflow.  There is a violet Side scatter (VSSC) optional configuration for nanoparticle resolution.

TECHNICAL INFORMATION
CytoFlex S Flow Analyzer 4L 13 Color, Beckman Coulter. Obtained 2018,
It is equipped with 13 colors/4 lasers and can analyze up to 96 parameters on a 96 – well plate. The fully activated instrument includes four channels from the 488 nm (Blue) laser, three from the 638 nm (Red) laser, four from the 405 nm (Violet) laser and two from the 808 nm (Infrared) laser.

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