2015
Publications Cellular Analysis
Mairinger, T.; Steiger, M.; Nocon, J.; Mattanovich, D.; Koellensperger, G.; Hann, S. (2015) Gas chromatography-quadrupole time-of-flight mass spectrometry-based determination of isotopologue and tandem mass isotopomer fractions of primary metabolites for 13C-metabolic flux analysis. Anal Chem 2015; 87(23):11792-802.
Neubauer, S.; Chu, DB.; Marx, H.; Sauer, M.; Hann, S.; Koellensperger, G. (2015) LC-MS/MS-based analysis of coenzyme A and short-chain acyl-coenzyme A thioesters. Anal Bioanal Chem 2015; 407(22):6681-8.
Rußmayer, H.; Buchetics, M.; Gruber, C.; Valli, M.; Grillitsch, K.; Modarres, G.; Guerrasio, R.; Klavins, K.; Neubauer, S.; Drexler, H.; Steiger, M.; Troyer, C.; Al Chalabi, A.; Krebiehl, G. , Sonntag, D.; Zellnig, G.; Daum, G.; Graf, AB.; Altmann, F.; Koellensperger, G.; Hann, S.; Sauer, M.; Mattanovich, D.; Gasser, B. (2015) Systems-level organization of yeast methylotrophic lifestyle. BMC Biol 2015.
Chu, DB.; Troyer, C.; Mairinger, T.; Ortmayr, K.; Neubauer, S.; Koellensperger, G.; Hann, S. (2015) Isotopologue analysis of sugar phosphates in yeast cell extracts by gas chromatography chemical ionization time-of-flight mass spectrometry. Anal Bioanal Chem 2015; 407(10):2865-75.
Hann, S.; Dernovics, M.; Koellensperger, G. (2015) Elemental analysis in biotechnology. Curr Opin Biotechnol 2015; 31:93-100.
Ortmayr, K.; Hann, S.; Koellensperger, G. (2015) Complementing reversed-phase selectivity with porous graphitized carbon to increase the metabolome coverage in an on-line two-dimensional LC-MS setup for metabolomics. Analyst 2015; 140(10):3465-73.
Russmayer, H.; Troyer, C.; Neubauer, S.; Steiger, MG.; Gasser, B.; Hann, S.; Koellensperger, G.; Sauer, M.; Mattanovich, D. (2015) Metabolomics sampling of pichia pastoris revisited: Rapid filtration prevents metabolite loss during quenching. FEMS Yeast Res 2015; 15(6).
Ortmayr, K.; Schwaiger, M.; Hann, S.; Koellensperger, G. (2015) An integrated metabolomics workflow for the quantification of sulfur pathway intermediates employing thiol protection with N-ethyl maleimide and hydrophilic interaction liquid chromatography tandem mass spectrometry. Analyst 2015; 140(22):7687-95.
Causon, T.J.; Hann, S. (2015) Theoretical evaluation of peak capacity improvements by use of liquid chromatography combined with drift tube ion mobility-mass spectrometry. J Chromatogr A 2015; 1416:47-56.
Boes, A.; Spiegel, H.; Edgue, G.; Kapelski, S.; Scheuermayer, M.; Fendel, R.; Remarque, E.; Altmann, F.; Maresch, D.; Reimann, A.; Pradel, G.; Schillberg, S.; Fischer, R. (2015) Detailed functional characterization of glycosylated and nonglycosylated variants of malaria vaccine candidate PfAMA1 produced in Nicotiana benthamiana and analysis of growth inhibitory responses in rabbits. Plant Biotechnol J. 13, 222-234.
Capone, S.; Ćorajević, L.; Bonifert, G.; Murth, P.; Maresch, D.; Altmann, F.; Herwig, C.; Spadiut, O. (2015) Combining Protein and Strain Engineering for the Production of Glyco-Engineered Horseradish Peroxidase C1A in Pichia pastoris. Int J Mol Sci. 16, 23127-23142.
Castilho, A.; Gruber, C.; Thader, A.; Oostenbrink, C.; Pechlaner, M.; Steinkellner, H.; Altmann, F. (2015) Processing of complex N-glycans in IgG Fc-region is affected by core fucosylation. MAbs 7, 863-870.
Friedrich, V.; Gruber, C.; Nimeth, I.; Pabinger, S.; Sekot, G.; Posch, G.; Altmann, F.; Messner, P.; Andrukhov, O.; Schäffer, C. (2015) Outer membrane vesicles of Tannerella forsythia: Biogenesis, composition, and virulence. Mol Oral Microbiol 30, 451-473.
Gmeiner, C.; Saadati, A.; Maresch, D.; Krasteva, S.; Frank, M.; Altmann, F.; Herwig, C.; Spadiut, O. (2015) Development of a fed-batch process for a recombinant Pichia pastoris ¿ och1 strain expressing a plant peroxidase. Microb Cell Fact. 14:1.
Gruber, C.; Altmann, F. (2015) Site-Specific Glycosylation Profiling Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS). Methods Mol Biol. 1321, 407-415.
Heiss, S.; Puxbaum, V.; Gruber, C.; Altmann, F.; Mattanovich, D.; Gasser, B. (2015) Multistep processing of the secretion leader of the extracellular protein Epx1 in Pichia pastoris and implications on protein localization. Microbiology 161, 1356-1368.
Hensel, G.; Floss, DM; Arcalis, E.; Sack, M.; Melnik, S.; Altmann, F.; Rutten, T.; Kumlehn, J.; Stoger, E.; Conrad, U. (2015) Transgenic Production of an Anti HIV Antibody in the Barley Endosperm. PLoS One. 10, e0140476.
Loos, A.; Gach, JS; Hackl, T.; Maresch, D.; Henkel, T.; Porodko, A.; Bui-Minh, D.; Sommeregger, W.; Wozniak-Knopp, G.; Forthal, DN; Altmann, F.; Steinkellner, H.; Mach, L. (2015) Glycan modulation and sulfoengineering of anti-HIV-1 monoclonal antibody PG9 in plants. Proc Natl Acad Sci U S A. 112, 12675-12680.
Ma, JK; Drossard, J.; Lewis, D.; Altmann, F.; Boyle, J.; Christou, P.; Cole, T.; Dale, P.; van Dolleweerd, CJ; Isitt, V.; Katinger, D.; Lobedan, M.; Mertens, H.; Paul, MJ; Rademacher, T.; Sack, M.; Hundleby, PA; Stiegler, G.; Stoger, E.; Twyman, RM; Vcelar, B.; Fischer, R. (2015) Regulatory approval and a first-in-human phase I clinical trial of a monoclonal antibody produced in transgenic tobacco plants. Plant Biotechnol J. 13, 1106-1120
Plattner, S.; Gruber, C.; Stadlmann, J.; Widmann, S.; Gruber, CW; Altmann, F.; Bohlmann, H. (2015) Isolation and Characterization of a Thionin Proprotein Processing Enzyme from Barley. J Biol Chem. 290, 18056-18067..
Rußmayer, H.; Buchetics, M.; Gruber, C.; Valli, M.; Grillitsch, K.; Modarres, G.; Guerrasio, R.; Klavins, K.; Neubauer, S., Drexler, H.; Steiger, M.; Troyer, C.; Al Chalabi, A.; Krebiehl, G.; Sonntag, D.; Zellnig, G.; Daum, G.; Graf, A.B.; Altmann, F.; Koellensperger, G.; Hann, S.; Sauer, M.; Mattanovich, D.; Gasser, B. (2015) Systems-level organization of yeast methylotrophic lifestyle. BMC Biology, 13, 80.
Schneider, J.; Castilho, A.; Pabst, M.; Altmann, F.; Gruber, C.; Strasser, R.; Gattinger, P.; Seifert, GJ; Steinkellner, H. (2015) Characterization of plants expressing the human β1,4-galactosyltrasferase gene. Plant Physiol Biochem. 92, 39-47
Chaudhuri, S.; Maurya, P.; Kauri, M.; Tiwari, A.; Borth, N.; Bhatnagar, S.; Kumar, N. (2015) Investigation of the CHO secretome: potential way to improve recombinant protein production from bioprocesses. J Bioprocessing Biotechn. 5:7
Harreither, E. ;Hackl, M.; Pichler, J.; Shridhar, S.; Auer, N.; Łabaj, P.P; Scheideler, M.; Karbiener, M.; Grillari, J.; Kreil, D.P.; Borth, N. (2015) Gene expression patterns of host cell subclones with increased production capacity. Biotechnol. J. 10, 1625-1638
Gasser, B.; Steiger, MG; Mattanovich, D. (2015) Methanol regulated yeast promoters: production vehicles and toolbox for synthetic biology. MICROB CELL FACT. 2015; 14:
Heiss, S.; Puxbaum, V.; Gruber, C.; Altmann, F.; Mattanovich, D.; Gasser, B. (2015) Multistep processing of the secretion leader of the extracellular protein Epx1 in Pichia pastoris and implications for protein localization. MICROBIOL-SGM. 2015; 161: 1356-1368.
Patzschke, A.; Steiger, MG; Holz, C.; Lang, C.; Mattanovich, D.; Sauer, M. (2015) Enhanced glutathione production by evolutionary engineering of Saccharomyces cerevisiae strains. Biotechnol J. 2015; 10(11):1719-1726
Prielhofer, R.; Cartwright, SP; Graf, AB; Valli, M.; Bill, RM; Mattanovich, D.; Gasser, B. (2015) Pichia pastoris regulates its gene-specific response to different carbon sources at the transcriptional, rather than the translational level. BMC Genomics. 2015; 16:167
Zámocký, M.; Hofbauer, S.; Schaffner, I.; Gasselhuber, B.; Nicolussi, A.; Soudi, M.; Pirker, KF; Furtmüller, PG; Obinger, C.; (2015) Independent evolution of four heme peroxidase superfamilies. Arch Biochem Biophys. 2015; 574:108-119
Fliedl, L.; Grillari, J.; Grillari-Voglauer, R.; (2015) Human cell lines for the production of recombinant proteins: on the horizon. N Biotechnol. 2015; 32(6):673-679
Fliedl, L.; Kast, F.; Grillari, J.; Wieser, M.; Grillari-Voglauer, R. (2015) Optimization of a quantitative PCR based method for plasmid copy number determination in human cell lines. New Biotechnol. 2015; 32(6):716-719
Schosserer, M.; Reynoso, R.; Wally, V.; Jug, B.; Kantner, V.; Weilner, S.; Buric, I.; Grillari, J.; Bauer, JW; Grillari-Voglauer, R.; (2015) Urine is a novel source of autologous mesenchymal stem cells for patients with epidermolysis bullosa. BMC Res Notes. 2015; 8(1):767
Chromikova, V.; Mader, A.; Steinfellner, W.; Kunert, R. (2015) Evaluating the bottlenecks of recombinant IgM production in mammalian cells. Cytotechnology. 2015; 67(2):343-356
Reinhart, D.; Damjanovic, L.; Kaisermayer, C.; Kunert, R. (2015) Benchmarking of commercially available CHO cell culture media for antibody production. APPL MICROBIOL BIOT. 2015; 99(11): 4645-4657.
Reinhart, D.; Kunert, R.; (2015) Upstream and downstream processing of recombinant IgA. Biotechnol Lett. 2015; 37(2):241-251
Zboray, K.; Sommeregger, W.; Bogner, E.; Gili, A.; Sterovsky, T.; Fauland, K.; Grabner, B.; Stiedl, P.; Moll, HP; Bauer, A.; Kunert, R.; Casanova, E.; (2015) Heterologous protein production using euchromatin-containing expression vectors in mammalian cells. Nucleic Acids Res. 2015; 43(16):e102
Detsch, R.; Alles, S.; Hum, J.; Westenberger, P.; Sieker, F.; Heusinger, D.; Kasper, C.; Boccaccini, AR (2015) Osteogenic differentiation of umbilical cord and adipose derived stem cells onto highly porous 45S5 Bioglass(®) -based scaffolds.. J Biomed Mater Res A. 2015; 103(3):1029-1037
Gugerell, A.; Neumann, A.; Kober, J.; Tammaro, L.; Hoch, E.; Schnabelrauch, M.; Kamolz, L.; Kasper, C.; Keck, M. (2015) Adipose-derived stem cells cultivated on electrospun l-lactide/glycolide copolymer fleece and gelatin hydrogels under flow conditions – aiming physiological reality in hypodermis tissue engineering. Burns. 2015; 41(1):163-171
Publications Extremohile Center
Tesei, D.; Marzban, G.; Marchetti-Deschmann, M.; Tafer, H.; Arcalis, E.; Sterflinger, K. (2015) Protein functional analysis data in support of comparative proteomics of the pathogenic black yeast Exophiala dermatitidis under different temperature conditions. 09/2015; 5. DOI:10.1016/j.dib.2015.08.008
Jroundi, F.; Gonzalez-Muñoz, T. M.; Sterflinger, K.; Piñar, G. (2015) Molecular Tools for Monitoring the Ecological Sustainability of a Stone Bio-Consolidation Treatment at the Royal Chapel, Granada. PLoS ONE 07/2015; 10(7):e0132465. DOI:10.1371/journal.pone.0132465
Tesei, D.; Marzban, G.; Marchetti-Deschmann, M.; Tafer, H.; Arcalis, E.; Sterflinger, K. (2015) Proteome of tolerance fine-tuning in the human pathogen black yeast Exophiala dermatitidis. Journal of proteomics 07/2015; 128. DOI:10.1016/j.jprot.2015.07.007
Piñar, G.; Tafer, H.; Sterflinger, K.; Pinzari, F. (2015) Amid the possible causes of a very famous foxing: Molecular and microscopic insight into Leonardo da Vinci’s self-portrait. Environmental Microbiology Reports 06/2015; 7(6). DOI:10.1111/1758-2229.12313
Blasi, B.; Tafer, H.; Tesei, D.; Sterflinger, K. (2015) From Glacier to Sauna: RNA-Seq of the Human Pathogen Black Fungus Exophiala dermatitidis under Varying Temperature Conditions Exhibits Common and Novel Fungal Response. PLoS ONE 06/2015; 10(6):e0127103. DOI:10.1371/journal.pone.0127103
Tafer, H.; Lopandic, K.; Blasi, B.; Poyntner, C.; Sterflinger, K. (2015) Draft Genome Sequence of Exophiala mesophila, a Black Yeast with High Bioremediation Potential. Genome Announcements 04/2015; 3(2). DOI:10.1128/genomeA.00203-15
Sterflinger, K.; Lopandic, K.; Blasi, B.; Poynter, C.; de Hoog, S.; Tafer, H. (2015) Draft Genome of Cladophialophora immunda, a Black Yeast and Efficient Degrader of Polyaromatic Hydrocarbons. Genome Announcements 01/2015; 3(1). DOI:10.1128/genomeA.01283-14
Kurzhals, S.; Zirbs, R.; Reimhult, E. (2015) Synthesis and magneto-thermal actuation of iron oxide core – PNIPAM shell nanoparticles, ACS Applied Materials & Interfaces, 7, 19342-19352, DOI: 10.1021/acsami.5b05459
Bixner, O.; Lassenberger, A.; Baurecht, D.; Reimhult, E. (2015) Complete exchange of hydrophobic dispersant shell on superpara-magnetic iron oxide nanoparticles, Langmuir, 31, 9198-9204, DOI: 10.1021/acs.langmuir.5b01833
Grünewald, T.A.; van Oostrum, P.D.J.; Lassenberger, A.; Zirbs, R.; Capone, B.; Vonderhaid, I.; Amenitsch, H.; Lichtenegger, H.C.; Reimhult, E. (2015) Core-shell structure of monodisperse PEG-grafted iron oxide nanoparticles studied by SAXS, Chemistry of Materials, 27, 4763-4771, DOI: 10.1021/acs.chemmater.5b01488
Zirbs, R.; Lassenberger, A.; Vonderhaid, I.; Kurzhals, S.; Reimhult, E. (2015) Melt-Grafting for the Synthesis of Core-Shell Nanoparticles with Ultra-High Dispersant Density, Nanoscale, 7, 11216-11225, DOI: 10.1039/C5NR02313K
Bellasio, M.; Mattanovich, D.; Sauer, M.; Marx, H. (2015) Organic acids from lignocellulose: Candida lignohabitans as a new microbial cell factory. Journal of Industrial Microbiology and Biotechnology, 42(5), 681-691.
Gasser, B.; Steiger, M. G.; Mattanovich, D. (2015) Methanol regulated yeast promoters: Production vehicles and toolbox for synthetic biology. Microbial Cell Factories, 14(1).
Patzschke, A.; Steiger, M. G.; Holz, C.; Lang, C.; Mattanovich, D.; Sauer, M. (2015) Enhanced glutathione production by evolutionary engineering of saccharomyces cerevisiae strains. Biotechnology Journal, 10(11), 1719-1726.
Rußmayer, H.; Buchetics, M.; Gruber, C.; Valli, M.; Grillitsch, K.; Modarres, G.; Gasser, B. (2015) Systems-level organization of yeast methylotrophic lifestyle. BMC Biology, 13(1).
Russmayer, H.; Troyer, C.; Neubauer, S.; Steiger, M. G.; Gasser, B.; Hann, S.; Mattanovich, D. (2015) Metabolomics sampling of Pichia pastoris revisited: Rapid filtration prevents metabolite loss during quenching. FEMS Yeast Research, 15(6).